Euthyroid testis-intact controls (INTACT) and hypothyroid-orquidectomized (TXOX) rats were performed as described in Material and Methods. Administration of E2 (TXOXE2) or vehicle (TXOX) to TXOX rats was performed for 20 days. Then, VEH (TXOX), E2 (TXOXE2), GH (TXOXGH) or E2 plus GH (TXOXE2GH) replacements were performed for additional 7 days. On PND90, animals were sacrificed and the liver extracted for fatty acid analyses as described in Material and Methods. Data are expressed as mean ± SEM for 5 independent samples (different animals) and each independent sample was tested twice. Values represent weight percent of total lipid. Values were submitted to ANOVA followed by post hoc Tukey's test. Values in the same row with different lowercase letters are significantly different with P<0.05.