Euthyroid testis-intact controls (INTACT) and hypothyroid-orquidectomized (TXOX) rats were performed as described in Material and Methods. Long-term administration of E2 (TXOXE2) or vehicle (VEH) to TXOX rats was performed for 20 days. Then, VEH (TXOX), E2 (TXOXE2), GH (TXOXGH) or E2 plus GH (TXOXE2GH) replacements were performed for additional 7 days. On PND90, the animals were sacrificed and serum and hepatic lipid classes were measured. Data are expressed as mean ± SEM for serum (n = 5) or hepatic (n = 5) independent samples (different animals) and each independent sample was tested twice. Values represent weight percent of total lipid. Values were submitted to ANOVA followed by post hoc Tukey's test. Values in the same row with different lowercase letters are significantly different with P<0.05.