Article open access publication

The Arabidopsis cytosolic proteome: the metabolic heart of the cell

Frontiers in Plant Science, Frontiers, ISSN 1664-462X

Volume 5, 2014

DOI:10.3389/fpls.2014.00021, Dimensions: pub.1019098059, PMC: PMC3914213, PMID: 24550929,

Authors

Ito, Jun (1) (2)
Parsons, Harriet T. (1) (2) (3)

Affiliations

Organisations

  1. (1) Joint BioEnergy Institute, grid.451372.6
  2. (2) Lawrence Berkeley National Laboratory, grid.184769.5
  3. (3) University of Copenhagen, grid.5254.6, KU

Description

The plant cytosol is the major intracellular fluid that acts as the medium for inter-organellar crosstalk and where a plethora of important biological reactions take place. These include its involvement in protein synthesis and degradation, stress response signaling, carbon metabolism, biosynthesis of secondary metabolites, and accumulation of enzymes for defense and detoxification. This central role is highlighted by estimates indicating that the majority of eukaryotic proteins are cytosolic. Arabidopsis thaliana has been the subject of numerous proteomic studies on its different subcellular compartments. However, a detailed study of enriched cytosolic fractions from Arabidopsis cell culture has been performed only recently, with over 1,000 proteins reproducibly identified by mass spectrometry. The number of proteins allocated to the cytosol nearly doubles to 1,802 if a series of targeted proteomic characterizations of complexes is included. Despite this, few groups are currently applying advanced proteomic approaches to this important metabolic space. This review will highlight the current state of the Arabidopsis cytosolic proteome since its initial characterization a few years ago.

Funders

Research Categories

Main Subject Area

Fields of Research

Links & Metrics

NORA University Profiles

University of Copenhagen

Dimensions Citation Indicators

Times Cited: 11

Field Citation Ratio (FCR): 1.37

Relative Citation ratio (RCR): 0.51

Open Access Info

Pure Gold