Article open access publication

Phylogenetic Analysis of Staphylococcus aureus CC398 Reveals a Sub-Lineage Epidemiologically Associated with Infections in Horses

PLoS ONE, Public Library of Science (PLoS), ISSN 1932-6203

Volume 9, 2, 2014

DOI:10.1371/journal.pone.0088083, Dimensions: pub.1021702774, PMC: PMC3913741, PMID: 24505386,



  1. (1) Robert Koch Institute, grid.13652.33
  2. (2) Free University of Berlin, grid.14095.39
  3. (3) State Serum Institute, grid.6203.7
  4. (4) Technical University of Denmark, grid.5170.3, DTU
  5. (5) University of Edinburgh, grid.4305.2
  6. (6) University of Iowa, grid.214572.7
  7. (7) Utrecht University, grid.5477.1
  8. (8) Istituto Superiore di Sanità, grid.416651.1
  9. (9) Centre National de Référence, Staphylococcus aureus, Microbiology Department, Erasme University Hospital, Université Libre de Bruxelles, Brussels, Belgium
  10. (10) European Centre for Disease Prevention and Control, grid.418914.1
  11. (11) Glasgow Royal Infirmary, grid.411714.6
  12. (12) Labor Dr. Böse GmbH, Harsum, Germany


In the early 2000s, a particular MRSA clonal complex (CC398) was found mainly in pigs and pig farmers in Europe. Since then, CC398 has been detected among a wide variety of animal species worldwide. We investigated the population structure of CC398 through mutation discovery at 97 genetic housekeeping loci, which are distributed along the CC398 chromosome within 195 CC398 isolates, collected from various countries and host species, including humans. Most of the isolates in this collection were received from collaborating microbiologists, who had preserved them over years. We discovered 96 bi-allelic polymorphisms, and phylogenetic analyses revealed that an epidemic sub-clone within CC398 (dubbed 'clade (C)') has spread within and between equine hospitals, where it causes nosocomial infections in horses and colonises the personnel. While clade (C) was strongly associated with S. aureus from horses in veterinary-care settings (p = 2 × 10(-7)), it remained extremely rare among S. aureus isolates from human infections.


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