Article open access publication

Jarid2 Is Implicated in the Initial Xist-Induced Targeting of PRC2 to the Inactive X Chromosome

Molecular Cell, Elsevier, ISSN 1097-2765

Volume 53, 2, 2014

DOI:10.1016/j.molcel.2014.01.002, Dimensions: pub.1025200005, PMID: 24462204,

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  1. (1) Curie Institute, CNRS UMR3215, INSERM U934, 26 rue d’Ulm, Paris 75248, France
  2. (2) Institute Curie, grid.418596.7
  3. (3) New York University, grid.137628.9
  4. (4) University of Copenhagen, grid.5254.6, KU
  5. (5) TU Dresden, grid.4488.0
  6. (6) ETH Zurich, grid.5801.c

Description

During X chromosome inactivation (XCI), the Polycomb Repressive Complex 2 (PRC2) is thought to participate in the early maintenance of the inactive state. Although Xist RNA is essential for the recruitment of PRC2 to the X chromosome, the precise mechanism remains unclear. Here, we demonstrate that the PRC2 cofactor Jarid2 is an important mediator of Xist-induced PRC2 targeting. The region containing the conserved B and F repeats of Xist is critical for Jarid2 recruitment via its unique N-terminal domain. Xist-induced Jarid2 recruitment occurs chromosome-wide independently of a functional PRC2 complex, unlike at other parts of the genome, such as CG-rich regions, where Jarid2 and PRC2 binding are interdependent. Conversely, we show that Jarid2 loss prevents efficient PRC2 and H3K27me3 enrichment to Xist-coated chromatin. Jarid2 thus represents an important intermediate between PRC2 and Xist RNA for the initial targeting of the PRC2 complex to the X chromosome during onset of XCI.

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University of Copenhagen

Danish Open Access Indicator

2014: Blocked

Research area: Science & Technology

Danish Bibliometrics Indicator

2014: Level 2

Research area: Science & Technology

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Times Cited: 163

Field Citation Ratio (FCR): 18.63

Relative Citation ratio (RCR): 4.76

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