Article open access publication

The Sinocyclocheilus cavefish genome provides insights into cave adaptation

BMC Biology, Springer Nature, ISSN 1478-5854

Volume 14, 1, 2016

DOI:10.1186/s12915-015-0223-4, Dimensions: pub.1026992084, PMC: PMC4698820, PMID: 26728391,

Authors

Bai, Jie (2) (3) (4)
Fang, Dongming (2) (5)
Qiu, Ying (2) (4) (6)
Yuan, Hui (2)
Bian, Chao (2) (4)
Lu, Jiang (2) (7)
He, Shiyang (2) (7)
Zhang, Yaolei (2) (8)
You, Xin Xin (2) (4)
Sun, Ying (2) (6)
Liu, Yong (2)
Zhang, He (2)
Zhang, Xinhui (2) (4)
Cheng, Le (6) (9)
Chen, Jieming (2) (4)
Ruan, Zhiqiang (2) (4)
Li, Jia (2) (4) (7)
Yu, Hui (2) (4) (7)
Peng, Chao (2) (4)
Ma, Xingyu (2) (10)
Xu, Junmin (2) (10)
He, You (11)
Xu, Pao (13)
Wang, Jian (2) (14)
Yang, Huan-Ming (2) (14)
Wang, Jun (2) (15)
Xu, Xun * (2)
Shi, Qiong * (2) (4) (10)

* Corresponding author

Affiliations

Organisations

  1. (1) Kunming Institute of Zoology, grid.419010.d
  2. (2) Beijing Genomics Institute, grid.21155.32
  3. (3) Fauna & Flora International, CB1 2JD, Cambridge, UK
  4. (4) Shenzhen Key Lab of Marine Genomics, State Key Laboratory of Agricultural Genomics, 518083, Shenzhen, China
  5. (5) Agricultural Genomics Institute at Shenzhen, grid.488316.0
  6. (6) China National Genebank, 518083, Shenzhen, China
  7. (7) University of Chinese Academy of Sciences, grid.410726.6
  8. (8) University of Electronic Science and Technology of China, grid.54549.39
  9. (9) BGI-Yunnan, 650106, Kunming, China
  10. (10) Zhenjiang BGI Fisheries Science & Technology Industrial Co. Ltd., 212000, Zhenjiang, China
  11. (11) Shanghai Institute of Applied Physics, grid.450275.1
  12. (12) Nanjing Medical University, grid.89957.3a
  13. (13) Chinese Academy of Fishery Sciences, grid.43308.3c
  14. (14) James D. Watson Institute of Genome Science, 310008, Hangzhou, China
  15. (15) University of Copenhagen, grid.5254.6, KU

Countries

China

Denmark

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Asia

Europe

Description

BACKGROUND: An emerging cavefish model, the cyprinid genus Sinocyclocheilus, is endemic to the massive southwestern karst area adjacent to the Qinghai-Tibetan Plateau of China. In order to understand whether orogeny influenced the evolution of these species, and how genomes change under isolation, especially in subterranean habitats, we performed whole-genome sequencing and comparative analyses of three species in this genus, S. grahami, S. rhinocerous and S. anshuiensis. These species are surface-dwelling, semi-cave-dwelling and cave-restricted, respectively. RESULTS: The assembled genome sizes of S. grahami, S. rhinocerous and S. anshuiensis are 1.75 Gb, 1.73 Gb and 1.68 Gb, respectively. Divergence time and population history analyses of these species reveal that their speciation and population dynamics are correlated with the different stages of uplifting of the Qinghai-Tibetan Plateau. We carried out comparative analyses of these genomes and found that many genetic changes, such as gene loss (e.g. opsin genes), pseudogenes (e.g. crystallin genes), mutations (e.g. melanogenesis-related genes), deletions (e.g. scale-related genes) and down-regulation (e.g. circadian rhythm pathway genes), are possibly associated with the regressive features (such as eye degeneration, albinism, rudimentary scales and lack of circadian rhythms), and that some gene expansion (e.g. taste-related transcription factor gene) may point to the constructive features (such as enhanced taste buds) which evolved in these cave fishes. CONCLUSION: As the first report on cavefish genomes among distinct species in Sinocyclocheilus, our work provides not only insights into genetic mechanisms of cave adaptation, but also represents a fundamental resource for a better understanding of cavefish biology.

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Times Cited: 101

Field Citation Ratio (FCR): 16.79

Relative Citation ratio (RCR): 6.97

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