Article open access publication

Intake of Hydrolyzed Casein is Associated with Reduced Body Fat Accretion and Enhanced Phase II Metabolism in Obesity Prone C57BL/6J Mice

PLoS ONE, Public Library of Science (PLoS), ISSN 1932-6203

Volume 10, 3, 2015

DOI:10.1371/journal.pone.0118895, Dimensions: pub.1029337243, PMC: PMC4349863, PMID: 25738501,

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Organisations

  1. (1) Aarhus University, grid.7048.b, AU
  2. (2) Fudan University, grid.8547.e
  3. (3) University of Copenhagen, grid.5254.6, KU
  4. (4) Norwegian Institute of Marine Research, grid.10917.3e

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Denmark

Norway

China

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Europe

Asia

Description

The amount and form of dietary casein have been shown to affect energy metabolism and lipid accumulation in mice, but the underlying mechanisms are not fully understood. We investigated 48 hrs urinary metabolome, hepatic lipid composition and gene expression in male C57BL/6J mice fed Western diets with 16 or 32 energy% protein in the form of extensively hydrolyzed or intact casein. LC-MS based metabolomics revealed a very strong impact of casein form on the urinary metabolome. Evaluation of the discriminatory metabolites using tandem mass spectrometry indicated that intake of extensively hydrolyzed casein modulated Phase II metabolism associated with an elevated urinary excretion of glucuronic acid- and sulphate conjugated molecules, whereas glycine conjugated molecules were more abundant in urine from mice fed the intact casein diets. Despite the differences in the urinary metabolome, we observed no differences in hepatic expression of genes involved in Phase II metabolism, but it was observed that expression of Abcc3 encoding ATP binding cassette c3 (transporter of glucuronic acid conjugates) was increased in livers of mice fed hydrolyzed casein. As glucuronic acid is derived from glucose and sulphate is derived from cysteine, our metabolomic data provided evidence for changes in carbohydrate and amino acid metabolism and we propose that this modulation of metabolism was associated with the reduced glucose and lipid levels observed in mice fed the extensively hydrolyzed casein diets.

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Aarhus University

University of Copenhagen

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Times Cited: 8

Field Citation Ratio (FCR): 1.15

Relative Citation ratio (RCR): 0.48

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