Article open access publication

Urinary loss of tricarboxylic acid cycle intermediates as revealed by metabolomics studies: an underlying mechanism to reduce lipid accretion by whey protein ingestion?

Journal of Proteome Research, American Chemical Society (ACS), ISSN 1535-3893

Volume 13, 5, 2014

DOI:10.1021/pr500039t, Dimensions: pub.1043745927, PMC: PMC4045150, PMID: 24702026,

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  1. (1) Norwegian Institute of Marine Research, grid.10917.3e
  2. (2) University of Copenhagen, grid.5254.6, KU
  3. (3) Aarhus University, grid.7048.b, AU
  4. (4) East China Normal University, grid.22069.3f

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Denmark

Norway

China

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Description

Whey protein intake is associated with the modulation of energy metabolism and altered body composition both in human subjects and in animals, but the underlying mechanisms are not yet elucidated. We fed obesity-prone C57BL/6J mice high-fat diets with either casein (HF casein) or whey (HF whey) for 6 weeks. At equal energy intake and apparent fat and nitrogen digestibility, mice fed HF whey stored less energy as lipids, evident both as lower white adipose tissue mass and as reduced liver lipids, compared with HF-casein-fed mice. Explorative analyses of 48 h urine, both by (1)H NMR and LC-MS metabolomic platforms, demonstrated higher urinary excretion of tricarboxylic acid (TCA) cycle intermediates citric acid and succinic acid (identified by both platforms), and cis-aconitic acid and isocitric acid (identified by LC-MS platform) in the HF whey, relative to in the HF-casein-fed mice. Targeted LC-MS analyses revealed higher citric acid and cis-aconitic acid concentrations in fed state plasma, but not in liver of HF-whey-fed mice. We propose that enhanced urinary loss of TCA cycle metabolites drain available substrates for anabolic processes, such as lipogenesis, thereby leading to reduced lipid accretion in HF-whey-fed compared to HF-casein-fed mice.

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University of Copenhagen

Aarhus University

Danish Open Access Indicator

2014: Blocked

Research area: Science & Technology

Danish Bibliometrics Indicator

2014: Level 2

Research area: Science & Technology

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Times Cited: 29

Field Citation Ratio (FCR): 3.61

Relative Citation ratio (RCR): 1.64

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