Article

Methicillin-resistant Staphylococcus aureus strains from Ghana include USA300

Journal of Global Antimicrobial Resistance, Elsevier, ISSN 2213-7165

Volume 3, 1, 2015

DOI:10.1016/j.jgar.2014.11.006, Dimensions: pub.1045249062, PMID: 27873646,

Authors

Egyir, Beverly * (1) (2) (3)
Monecke, Stefan (4) (5)

* Corresponding author

Affiliations

Organisations

  1. (1) University of Copenhagen, grid.5254.6, KU
  2. (2) Noguchi Memorial Institute for Medical Research, grid.462644.6
  3. (3) State Serum Institute, grid.6203.7
  4. (4) Alere (Germany), grid.472845.8
  5. (5) TU Dresden, grid.4488.0
  6. (6) University of Ghana, grid.8652.9

Countries

Ghana

Denmark

Germany

Continents

Africa

Europe

Description

The objective of this study was to provide baseline information on circulating methicillin-resistant Staphylococcus aureus (MRSA) clones in Ghana. Thirty MRSA isolates collected between 2010 and 2013 from patients and healthy carriers were characterised by DNA microarray analysis, staphylococcal protein A (spa) typing, multilocus sequence typing (MLST) and minimum inhibitory concentration (MIC) determination to 21 antimicrobial agents. Phenotypic resistance was detected to tetracycline (67%), norfloxacin (40%), moxifloxacin (37%), erythromycin (37%), clindamycin (33%), gentamicin (30%), kanamycin (30%) and ceftaroline (20%), whereas no resistance was observed for glycopeptides, linezolid, daptomycin and tigecycline. DNA microarray analysis showed that tet(M) (43%), tet(K) (33%), aphA3 (23%), aacA-aphD (17%) and erm(C) (13%) were the most prevalent resistance genes. ST88-IV (WA MRSA-2) (n=8), ST8-IV (USA300) (n=5) containing arginine catabolic mobile element (ACME) and Panton-Valentine leukocidin (PVL), and ST247-I (North German/Iberian EMRSA) (n=4) were the most frequent clones detected. All MRSA contained sak and scn genes, one isolate (ST36-II) harboured the gene encoding the toxic shock syndrome toxin (TSST) and none contained exfoliative toxin genes. In conclusion, the relatively high levels of resistance to easily accessible non-β-lactam agents further complicate the treatment of MRSA infections in Ghana. The occurrence of USA300 and other epidemic multidrug-resistant MRSA clones in this African country is a matter of public health concern due to the lack of adequate infrastructures for MRSA surveillance and control in this geographical setting.

Research Categories

Main Subject Area

Fields of Research

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NORA University Profiles

University of Copenhagen

Danish Open Access Indicator

2015: Blocked

Research area: Medicine

Dimensions Citation Indicators

Times Cited: 17

Field Citation Ratio (FCR): 6.29

Relative Citation ratio (RCR): 1.28