Article

Effect of plant proteins and crystalline amino acid supplementation on postprandial plasma amino acid profiles and metabolic response in rainbow trout (Oncorhynchus mykiss)

Aquaculture International, Springer Nature, ISSN 0967-6120

Volume 23, 4, 2015

DOI:10.1007/s10499-014-9865-4, Dimensions: pub.1047969848,

Authors

Rolland, Marine * (1) (2)

* Corresponding author

Affiliations

Organisations

  1. (1) Technical University of Denmark, grid.5170.3, DTU
  2. (2) BioMar (Denmark), grid.424148.f

Countries

Denmark

Continents

Europe

Description

The use of aquafeeds formulated with plant protein sources supplemented with crystalline amino acids (CAAs) is believed to influence amino acid (AA) uptake patterns and AA metabolic fate. Oxygen consumption and ammonia excretion rates were measured in rainbow trout (468.5 ± 86.5 g) force fed 0.75 % of their body mass with a diet based on either (1) fish meal (FM), (2) pea protein concentrate (PPC), or (3) pea protein concentrate supplemented with histidine, lysine, methionine and threonine (PPC+) to mimic FM AA profile. The specific dynamic action and nitrogen quotient (NQ) were calculated for 48 h of the postprandial period. In parallel, plasma AA concentrations were measured in blood samples withdrawn from the caudal vein before and then 2, 4, 6, 8, 12, 20, 32 and 48 h after feed administration. The unbalanced diet PPC had a significantly higher NQ compared to FM (0.29 ± 0.09 and 0.18 ± 0.04, respectively), and plasma profiles of essential AAs reflected the dietary deficiencies. Supplementation with CAA in diet PPC+ resulted in an intermediary NQ (0.21 ± 0.04) and significantly affected plasma AA profiles, presenting greater and faster rises followed by sharp decreases compared to FM. The strongest effect was observed for methionine, presenting threefold higher concentrations at peak time for PPC+ compared to FM (297.0 ± 77.0 and 131.8 ± 39.0 nmol ml−1, respectively). The differences in AA availability and metabolic profile in the pea diets compared to the FM diet were believed to be caused by an unbalanced dietary AA profile and CAA supplementation, rather than inclusion of plant protein concentrate.

Research Categories

Main Subject Area

Links & Metrics

NORA University Profiles

Technical University of Denmark

Danish Open Access Indicator

2015: Unused

Research area: Science & Technology

Danish Bibliometrics Indicator

2015: Level 1

Research area: Science & Technology

Dimensions Citation Indicators

Times Cited: 12

Field Citation Ratio (FCR): 2.37