Article open access publication

Mte1 interacts with Mph1 and promotes crossover recombination and telomere maintenance

Genes & Development, Cold Spring Harbor Laboratory, ISSN 0890-9369

Volume 30, 6, 2016

DOI:10.1101/gad.276204.115, Dimensions: pub.1048805999, PMC: PMC4803055, PMID: 26966248,

Affiliations

Organisations

  1. (1) University of Copenhagen, grid.5254.6, KU
  2. (2) Masaryk University, grid.10267.32
  3. (3) Institute of Molecular Biology, grid.424631.6
  4. (4) International Clinical Research Center of St. Anne's University Hospital Brno, grid.483343.b
  5. (5) University Hospital Brno, grid.412554.3

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Czech Republic

Denmark

Germany

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Europe

Description

Mph1 is a member of the conserved FANCM family of DNA motor proteins that play key roles in genome maintenance processes underlying Fanconi anemia, a cancer predisposition syndrome in humans. Here, we identify Mte1 as a novel interactor of the Mph1 helicase in Saccharomyces cerevisiae. In vitro, Mte1 (Mph1-associated telomere maintenance protein 1) binds directly to DNA with a preference for branched molecules such as D loops and fork structures. In addition, Mte1 stimulates the helicase and fork regression activities of Mph1 while inhibiting the ability of Mph1 to dissociate recombination intermediates. Deletion of MTE1 reduces crossover recombination and suppresses the sensitivity of mph1Δ mutant cells to replication stress. Mph1 and Mte1 interdependently colocalize at DNA damage-induced foci and dysfunctional telomeres, and MTE1 deletion results in elongated telomeres. Taken together, our data indicate that Mte1 plays a role in regulation of crossover recombination, response to replication stress, and telomere maintenance.

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University of Copenhagen

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Times Cited: 20

Field Citation Ratio (FCR): 3.21

Relative Citation ratio (RCR): 0.88

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Hybrid