Article open access publication

1H-13C NMR-Based Profiling of Biotechnological Starch Utilization.

Analytical Chemistry, American Chemical Society (ACS), ISSN 0003-2700

Volume 88, 19, 2016

DOI:10.1021/acs.analchem.6b02555, Dimensions: pub.1055080276, PMID: 27602592,

Affiliations

Organisations

  1. (1) Aarhus University, grid.7048.b, AU
  2. (2) Technical University of Denmark, grid.5170.3, DTU

Countries

Denmark

Continents

Europe

Description

Starch is used in food- and nonfood applications as a renewable and degradable source of carbon and energy. Insight into the chemical detail of starch degradation remains challenging as the starch constituents amylose and amylopectin are homopolymers. We show that considerable molecular detail of starch fragmentation can be obtained from multivariate analysis of spectral features in optimized 1H-13C NMR spectroscopy of starch fragments to identify relevant features that distinguish processes in starch utilization. As a case study, we compare the profiles of starch fragments in commercial beer samples. Spectroscopic profiles of homooligomeric starch fragments can be excellent indicators of process conditions. In addition, differences in the structure and composition of starch fragments have predictive value for downstream process output such as ethanol production from starch. Thus, high-resolution 1H-13C NMR spectroscopic profiles of homooligomeric fragment mixtures in conjunction with chemometric methods provide a useful addition to the analytical chemistry toolbox of biotechnological starch utilization.

Funders

Research Categories

Main Subject Area

Fields of Research

Links & Metrics

NORA University Profiles

Aarhus University

Technical University of Denmark

Danish Open Access Indicator

2016: Realized

Research area: Science & Technology

Danish Bibliometrics Indicator

2016: Level 2

Research area: Science & Technology

Dimensions Citation Indicators

Times Cited: 3

Field Citation Ratio (FCR): 0.49

Relative Citation ratio (RCR): 0.36

Open Access Info

Green, Submitted