Article

Profiling the Hydrolysis of Isolated Grape Berry Skin Cell Walls by Purified Enzymes.

Journal of Agricultural and Food Chemistry, American Chemical Society (ACS), ISSN 0021-8561

Volume 63, 37, 2015

DOI:10.1021/acs.jafc.5b02847, Dimensions: pub.1055095210, PMID: 26309153,

Affiliations

Organisations

  1. (1) Stellenbosch University, grid.11956.3a
  2. (2) University of Copenhagen, grid.5254.6, KU

Countries

South Africa

Denmark

Continents

Africa

Europe

Description

The unraveling of crushed grapes by maceration enzymes during winemaking is difficult to study because of the complex and rather undefined nature of both the substrate and the enzyme preparations. In this study we simplified both the substrate, by using isolated grape skin cell walls, and the enzyme preparations, by using purified enzymes in buffered conditions, to carefully follow the impact of the individual and combined enzymes on the grape skin cell walls. By using cell wall profiling techniques we could monitor the compositional changes in the grape cell wall polymers due to enzyme activity. Extensive enzymatic hydrolysis, achieved with a preparation of pectinases or pectinases combined with cellulase or hemicellulase enzymes, completely removed or drastically reduced levels of pectin polymers, whereas less extensive hydrolysis only opened up the cell wall structure and allowed extraction of polymers from within the cell wall layers. Synergistic enzyme activity was detectable as well as indications of specific cell wall polymer associations.

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University of Copenhagen

Danish Open Access Indicator

2015: Blocked

Research area: Science & Technology

Danish Bibliometrics Indicator

2015: Level 2

Research area: Science & Technology

Dimensions Citation Indicators

Times Cited: 15

Relative Citation ratio (RCR): 0.87