Article

Probing interactions between β-glucan and bile salts at atomic detail by ¹H-¹³C NMR assays.

Journal of Agricultural and Food Chemistry, American Chemical Society (ACS), ISSN 0021-8561

Volume 62, 47, 2014

DOI:10.1021/jf504352w, Dimensions: pub.1055914870, PMID: 25375023,

Affiliations

Organisations

  1. (1) University of Copenhagen, grid.5254.6, KU
  2. (2) Carlsberg Laboratory, grid.418674.8
  3. (3) University of Manchester, grid.5379.8
  4. (4) Technical University of Denmark, grid.5170.3, DTU

Countries

Denmark

United Kingdom

Continents

Europe

Description

Polysaccharides are prospective hosts for the delivery and sequestration of bioactive guest molecules. Polysaccharides of dietary fiber, specifically cereal (1 → 3)(1 → 4)-β-glucans, play a role in lowering the blood plasma cholesterol level in humans. Direct host-guest interactions between β-glucans and conjugated bile salts are among the possible molecular mechanisms explaining the hypocholesterolemic effects of β-glucans. The present study shows that (1)H-(13)C NMR assays on a time scale of minutes detect minute signal changes in both bile salts and β-glucans, thus indicating dynamic interactions between bile salts and β-glucans. Experiments are consistent with stronger interactions at pH 5.3 than at pH 6.5 in this in vitro assay. The changes in bile salt and β-glucan signals suggest a stabilization of bile salt micelles and concomitant conformational changes in β-glucans.

Funders

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NORA University Profiles

University of Copenhagen

Technical University of Denmark

Danish Open Access Indicator

2014: Blocked

Research area: Science & Technology

Danish Bibliometrics Indicator

2014: Level 2

Research area: Science & Technology

Dimensions Citation Indicators

Times Cited: 9

Relative Citation ratio (RCR): 0.4