Article

Optimizing sampling strategies for NMR-based metabolomics of human feces: pooled vs. unpooled analyses

Analytical Methods, Royal Society of Chemistry (RSC), ISSN 1759-9679

Volume 9, 30, 2017

DOI:10.1039/c7ay01465a, Dimensions: pub.1090740500,

Authors

Lamichhane, Santosh (1) (2) (3)
Bl├Ždel, Trine (1) (4) (5) (6)
Dalsgaard, Trine K. (1) (2) (3)
Larsen, Lesli H. (1) (4) (5) (6)
Dragsted, Lars O. (1) (4) (5) (6)
Astrup, Arne (1) (4) (5) (6)
Bertram, Hanne C. (1) (2) (3)

Affiliations

Organisations

  1. (1) Denmark
  2. (2) Aarhus University, grid.7048.b, AU
  3. (3) Department of Food Science
  4. (4) DK-2200 Copenhagen N
  5. (5) Department of Nutrition Exercise and Sports
  6. (6) University of Copenhagen, grid.5254.6, KU

Countries

Denmark

Continents

Europe

Description

This study focused on elucidating fecal sample collection for 1 H NMR-based metabolite profiling.

This study focused on elucidating fecal sample collection for 1 H NMR-based metabolite profiling. Pooled vs. unpooled feces sampling was compared to determine if single unpooled samples are adequate and representative for NMR based metabolomics of feces. Our results show that the metabolome of unpooled fecal samples varied within the individual (day-to-day variation). This variation could mainly be ascribed to fluctuations (CV > 40%) in the level of the metabolites glucose, lactate, and succinate. Consequently, our data suggest that multiple sampling and pooling of fecal samples could be considered to minimize errors arising from day to day variation in fecal sample.

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NORA University Profiles

Aarhus University

University of Copenhagen

Danish Open Access Indicator

2017: Unused

Research area: Science & Technology

Danish Bibliometrics Indicator

2017: Level 1

Research area: Science & Technology

Dimensions Citation Indicators

Times Cited: 11

Field Citation Ratio (FCR): 3.04