Article open access publication

Undefined cellulase formulations hinder scientific reproducibility

Biotechnology for Biofuels, Springer Nature, ISSN 1754-6834

Volume 10, 1, 2017

DOI:10.1186/s13068-017-0974-y, Dimensions: pub.1093060431, PMC: PMC5704559, PMID: 29209415,

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  1. (1) National Renewable Energy Laboratory, grid.419357.d
  2. (2) University of Illinois at Urbana Champaign, grid.35403.31
  3. (3) Weizmann Institute of Science, grid.13992.30
  4. (4) University of Copenhagen, grid.5254.6, KU
  5. (5) University of California, Davis, grid.27860.3b
  6. (6) University of California, Riverside, grid.266097.c
  7. (7) Megazyme, Irishtown, Bray, A98 YV29, Co. Wicklow, Ireland
  8. (8) Rhodes University, grid.91354.3a

Description

In the shadow of a burgeoning biomass-to-fuels industry, biological conversion of lignocellulose to fermentable sugars in a cost-effective manner is key to the success of second-generation and advanced biofuel production. For the effective comparison of one cellulase preparation to another, cellulase assays are typically carried out with one or more engineered cellulase formulations or natural exoproteomes of known performance serving as positive controls. When these formulations have unknown composition, as is the case with several widely used commercial products, it becomes impossible to compare or reproduce work done today to work done in the future, where, for example, such preparations may not be available. Therefore, being a critical tenet of science publishing, experimental reproducibility is endangered by the continued use of these undisclosed products. We propose the introduction of standard procedures and materials to produce specific and reproducible cellulase formulations. These formulations are to serve as yardsticks to measure improvements and performance of new cellulase formulations.

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University of Copenhagen

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Times Cited: 5

Field Citation Ratio (FCR): 1.32

Relative Citation ratio (RCR): 0.53

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