Article open access publication

Technical challenges of working with extracellular vesicles

Nanoscale, Royal Society of Chemistry (RSC), ISSN 2040-3372

Volume 10, 3, 2018

DOI:10.1039/c7nr08360b, Dimensions: pub.1099657867, PMID: 29265147,



  1. (1) Federal University of Paraná, grid.20736.30
  2. (2) Oswaldo Cruz Foundation, grid.418068.3
  3. (3) AC Camargo Hospital, grid.413320.7
  4. (4) University of Nottingham, grid.4563.4
  5. (5) Aston University, grid.7273.1
  6. (6) University of Southampton, grid.5491.9
  7. (7) Universidade de São Paulo, grid.11899.38
  8. (8) University of Gothenburg, grid.8761.8
  9. (9) University of Oxford, grid.4991.5
  10. (10) University of St Andrews, grid.11914.3c
  11. (11) Federal University of Sao Paulo, grid.411249.b
  12. (12) London Metropolitan University, grid.23231.31
  13. (13) Universidade Anhanguera de São Paulo, São Paulo, Brazil.
  14. (14) University of Southern Denmark, grid.10825.3e, SDU
  15. (15) Technical University of Munich, grid.6936.a
  16. (16) University of Glasgow, grid.8756.c
  17. (17) Oxford Brookes University, grid.7628.b
  18. (18) Fondazione Santa Lucia, grid.417778.a


Extracellular Vesicles (EVs) are gaining interest as central players in liquid biopsies, with potential applications in diagnosis, prognosis and therapeutic guidance in most pathological conditions. These nanosized particles transmit signals determined by their protein, lipid, nucleic acid and sugar content, and the unique molecular pattern of EVs dictates the type of signal to be transmitted to recipient cells. However, their small sizes and the limited quantities that can usually be obtained from patient-derived samples pose a number of challenges to their isolation, study and characterization. These challenges and some possible options to overcome them are discussed in this review.


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Research area: Medicine

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Times Cited: 157

Relative Citation ratio (RCR): 16.5

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