Article open access publication

YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration

Cell Stem Cell, Elsevier, ISSN 1875-9777

Volume 22, 1, 2017

DOI:10.1016/j.stem.2017.11.001, Dimensions: pub.1099672256, PMC: PMC5766831, PMID: 29249464,

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  1. (1) University of Copenhagen, grid.5254.6, KU
  2. (2) University of Padua, grid.5608.b
  3. (3) Wellcome / MRC Cambridge Stem Cell Institute, grid.449973.4
  4. (4) Herlev Hospital, grid.411900.d, Capital Region
  5. (5) Lund University, grid.4514.4
  6. (6) Tokyo Medical and Dental University, grid.265073.5

Description

Tissue regeneration requires dynamic cellular adaptation to the wound environment. It is currently unclear how this is orchestrated at the cellular level and how cell fate is affected by severe tissue damage. Here we dissect cell fate transitions during colonic regeneration in a mouse dextran sulfate sodium (DSS) colitis model, and we demonstrate that the epithelium is transiently reprogrammed into a primitive state. This is characterized by de novo expression of fetal markers as well as suppression of markers for adult stem and differentiated cells. The fate change is orchestrated by remodeling the extracellular matrix (ECM), increased FAK/Src signaling, and ultimately YAP/TAZ activation. In a defined cell culture system recapitulating the extracellular matrix remodeling observed in vivo, we show that a collagen 3D matrix supplemented with Wnt ligands is sufficient to sustain endogenous YAP/TAZ and induce conversion of cell fate. This provides a simple model for tissue regeneration, implicating cellular reprogramming as an essential element.

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University of Copenhagen

Danish Open Access Indicator

2018: Realized

Research area: Medicine

Danish Bibliometrics Indicator

2018: Level 3

Research area: Medicine

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Times Cited: 181

Field Citation Ratio (FCR): 30.81

Relative Citation ratio (RCR): 11.19

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