Article open access publication

Multicentre Determination of Rezafungin (CD101) Susceptibility of Candida species by the EUCAST Method

Clinical Microbiology and Infection, Elsevier, ISSN 1198-743X

Volume 24, 11, 2018

DOI:10.1016/j.cmi.2018.02.021, Dimensions: pub.1101321208, PMID: 29505881,

Affiliations

Organisations

  1. (1) Rigshospitalet, grid.475435.4, Capital Region
  2. (2) University of Copenhagen, grid.5254.6, KU
  3. (3) State Serum Institute, grid.6203.7
  4. (4) National and Kapodistrian University of Athens, grid.5216.0
  5. (5) Erasmus University Medical Center, grid.5645.2
  6. (6) Mycology Reference Laboratory, National Centre for Microbiology, Instituto de Salud Carlos III. Carretera Majadahonda-Pozuelo, Km2. Majadahonda 28220. Madrid, Spain
  7. (7) Hospital General Universitario Gregorio Marañón, grid.410526.4
  8. (8) Institute of Health Carlos III, grid.413448.e

Countries

Netherlands

Greece

Denmark

Spain

Continents

Europe

Description

OBJECTIVES: Rezafungin (CD101) is a new long-acting echinocandin allowing weekly dosing, currently undergoing phase-II clinical trials for invasive candidiasis. The aim of this study was to assess rezafungin's in vitro activity against the most frequent Candida species following the EUCAST methodology. METHODS: The susceptibility of 2018 clinical Candida isolates was determined at four European laboratories. In parallel, six control strains were repeatedly tested. Wild-type upper limits (WT-ULs), defined as the MIC value where the wild-type distribution ends, were determined following the principles for EUCAST ECOFF-setting. RESULTS: The lowest rezafungin MICs (geometric MIC (GM-MIC), MIC range (mg/L)) were observed for C. albicans (0.016, 0.002-0.125) and the highest for C. parapsilosis (1.657, 0.063->4). MICs for the remaining species were in between these values (GM-MICs 0.048-0.055). Visual and statistical WT-ULs were identical for C. glabrata (0.125), C. krusei (0.125), C. parapsilosis (4), and C. tropicalis (0.25). If adopting these WT-ULs for classification into WT and non-WT populations, 1/413 C. glabrata, 1/402 C. krusei, 1/398 C. parapsilosis, and 1/402 C. tropicalis isolates were categorized as non-WT, all of which derived from Laboratory 1. For C. albicans unexplained laboratory variation was observed (WT-UL: 0.063-0.125 in Laboratories 1 and 2 versus 0.016 in Laboratories 3 and 4). A similar systematic difference was observed comparing the MICs for the three C. albicans QC strains, specifically, obtained in Laboratories 1and 2 with those in Laboratories 3 and 4. DISCUSSION: Rezafungin displayed species-specific activity similar to other echinocandins. Interlaboratory variation was observed for the most susceptible species C. albicans clinical and QC strains, an observation that warrants further investigation.

Research Categories

Main Subject Area

Fields of Research

Links & Metrics

NORA University Profiles

University of Copenhagen

Danish Open Access Indicator

2018: Unused

Research area: Medicine

Danish Bibliometrics Indicator

2018: Level 1

Research area: Medicine

Dimensions Citation Indicators

Times Cited: 13

Field Citation Ratio (FCR): 9.26

Relative Citation ratio (RCR): 3.36

Open Access Info

Hybrid