Real-time monitoring of enzyme-assisted animal protein hydrolysis by NMR spectroscopy – An NMR reactomics concept

LWT, Elsevier, ISSN 1096-1127

Volume 95, 2018

DOI:10.1016/j.lwt.2018.04.055, Dimensions: pub.1103593269,


* Corresponding author



  1. (1) Aarhus University, grid.7048.b, AU






This study presents an application of proton (1H) NMR spectroscopy for monitoring of enzyme-assisted hydrolysis of muscle protein under both at-line and real-time conditions. Measurements were carried out on sample material >1 g, and two different enzymes (alcalase and papain) were examined. The real-time monitoring of reactions was carried out directly in the NMR spectrometer, and the technique enabled the dynamic and quantitative detection of a total of 40 metabolites. The approach enabled to follow time evolution and kinetic modelling allowed extraction of kinetic parameters; a was calculated to 0.051 min−1 and 0.029 min−1 for alcalase and papain, respectively, revealing a higher hydrolysis velocity for alcalase than papain. The study demonstrates that NMR spectroscopy is an excellent analytical platform for real-time monitoring of the major metabolite composition changes occurring during an enzymatic hydrolysis. Industrial relevance: Protein hydrolysis is a commonly applied technology in food industry and the presented NMR methodology for kinetic studies of metabolite generation during a hydrolysis process would be applicable for testing and optimization of different raw materials and processing conditions.


Research Categories

Links & Metrics

NORA University Profiles

Aarhus University

Danish Open Access Indicator

2018: Unused

Research area: Science & Technology

Danish Bibliometrics Indicator

2018: Level 1

Research area: Science & Technology

Dimensions Citation Indicators

Times Cited: 5

Field Citation Ratio (FCR): 1.71