Article open access publication

A low-gluten diet induces changes in the intestinal microbiome of healthy Danish adults

Nature Communications, Springer Nature, ISSN 2041-1723

Volume 9, 1, 2018

DOI:10.1038/s41467-018-07019-x, Dimensions: pub.1107954773, PMC: PMC6234216, PMID: 30425247,



  1. (1) Technical University of Denmark, grid.5170.3, DTU
  2. (2) University of Copenhagen, grid.5254.6, KU
  3. (3) Flanders Institute for Biotechnology, grid.11486.3a
  4. (4) Rega Institute for Medical Research, grid.415751.3
  5. (5) University of Southern Denmark, grid.10825.3e, SDU
  6. (6) Bispebjerg Hospital, grid.411702.1, Capital Region
  7. (7) Hvidovre Hospital, grid.411905.8, Capital Region
  8. (8) State Serum Institute, grid.6203.7
  9. (9) Chalmers University of Technology, grid.5371.0
  10. (10) University of Auckland, grid.9654.e
  11. (11) Rigshospitalet, grid.475435.4, Capital Region
  12. (12) Research Centre for Prevention and Health, The Capital Region of Denmark, DK-2000, Frederiksberg, Denmark
  13. (13) Gentofte Hospital, grid.411646.0, Capital Region
  14. (14) Clinical-Microbiomics A/S, DK-2200, Copenhagen, Denmark


Adherence to a low-gluten diet has become increasingly common in parts of the general population. However, the effects of reducing gluten-rich food items including wheat, barley and rye cereals in healthy adults are unclear. Here, we undertook a randomised, controlled, cross-over trial involving 60 middle-aged Danish adults without known disorders with two 8-week interventions comparing a low-gluten diet (2 g gluten per day) and a high-gluten diet (18 g gluten per day), separated by a washout period of at least six weeks with habitual diet (12 g gluten per day). We find that, in comparison with a high-gluten diet, a low-gluten diet induces moderate changes in the intestinal microbiome, reduces fasting and postprandial hydrogen exhalation, and leads to improvements in self-reported bloating. These observations suggest that most of the effects of a low-gluten diet in non-coeliac adults may be driven by qualitative changes in dietary fibres.


Research Categories

Main Subject Area

Fields of Research

Links & Metrics

NORA University Profiles

Technical University of Denmark

University of Copenhagen

University of Southern Denmark

Danish Open Access Indicator

2018: Realized

Research area: Science & Technology

Danish Bibliometrics Indicator

2018: Level 2

Research area: Science & Technology

Dimensions Citation Indicators

Times Cited: 21

Field Citation Ratio (FCR): 11.52

Relative Citation ratio (RCR): 1.5

Open Access Info

Pure Gold