Article

A high-throughput screen identifies the long non-coding RNA DRAIC as a regulator of autophagy

Oncogene, Springer Nature, ISSN 0950-9232

Volume 38, 26, 2019

DOI:10.1038/s41388-019-0783-9, Dimensions: pub.1112768917, PMID: 30872794,

Affiliations

Organisations

  1. (1) University of Copenhagen, grid.5254.6, KU
  2. (2) Danish Cancer Society, grid.417390.8
  3. (3) Division of Cancer Research, Department of Thoracic Surgery, Medical Center—University of Freiburg, Faculty of Medicine, 79106, Freiburg, Germany
  4. (4) German Cancer Research Center, grid.7497.d
  5. (5) University of Freiburg, grid.5963.9

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Germany

Denmark

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Europe

Description

Autophagy is a conserved degradation process that occurs in all eukaryotic cells and its dysfunction has been associated with various diseases including cancer. While a number of large-scale attempts have recently identified new molecular players in autophagy regulation, including proteins and microRNAs, little is known regarding the function of long non-coding RNAs (lncRNAs) in the regulation of this process. To identify new long non-coding RNAs with functional implications in autophagy, we performed a high-throughput RNAi screen targeting more than 600 lncRNA transcripts and monitored their effects on autophagy in MCF-7 cells. We identified 63 lncRNAs that affected GFP-LC3B puncta numbers significantly. We validated the strongest hit, the lncRNA DRAIC previously shown to impact cell proliferation, and revealed a novel role for this lncRNA in the regulation of autophagic flux. Interestingly, we find DRAIC's pro-proliferative effects to be autophagy-independent. This study serves as a valuable resource for researchers from both the lncRNA and autophagy fields as it advances the current understanding of autophagy regulation by non-coding RNAs.

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NORA University Profiles

University of Copenhagen

Danish Open Access Indicator

2019: Unused

Research area: Medicine

Danish Bibliometrics Indicator

2019: Level 1

Research area: Medicine

Dimensions Citation Indicators

Times Cited: 15

Field Citation Ratio (FCR): 10.89

Relative Citation ratio (RCR): 1.34